GEPIA platform were used to validate of the mRNA expression of 9 HOUP hub genes, named TNF, UBC, SRC, ESR1, CDK1, PECAM1, CXCR4, MUC1 and IKBKG, with data from TCGA and GTEx, TNF, ESR1, CDK1, CXCR4 and MUC1 were confirmed significantly higher in ovarian cancer samples than that in normal samples. The gene discussed is TNF; the disease is ovarian cancer.