We further analysed the levels of Tsc1 and Tsc2 mRNA in diabetic nephropathy using the Nephroseq database (https://www.nephroseq.org) and found that Tsc2 mRNA was also significantly decreased in both glomeruli (Glom) and the tubulointerstitium (TubInt) from patients with diabetic nephropathy (Glom in healthy living donor: 0.92 ± 0.31; Glom in diabetic nephropathy: 0.71 ± 0.46 [P = 0.002]; TubInt in healthy living donor: 0.13 ± 0.24; TubInt in diabetic nephropathy: -0.02 ± 0.27 [P = 0.02]). This evidence concerns the gene TSC1 and diabetic kidney disease.