However, after 48 h of erlotinib treatment, cleaved caspase-3 was clearly detectable in cells harboring L858R and also detectable at lesser extent in cells harboring EGFR WT and in most cells harboring HCC-derived EGFR mutants but still not detectable in cells harboring T790M (Fig. 7b). Here, CASP3 is linked to hepatocellular carcinoma.