In order to confirm the therapeutic effect of the recombinant anti-AEG-1 ScFv adenoviruses, we carried out in vivo tumor growth inhibition experiment in nude mice After 4 weeks of inoculation of HeLa cells and treatment with Ad-sta-SFv, we measured tumor size and found that the average size and weight of the xenografts formed from HeLa/Ad-sta-SFv group (averaged size/weight inhibit rate 53.98% and 37.48%, respectively) was obviously smaller than that of the xenografts formed from control groups (Fig. 5b). This evidence concerns the gene MTDH and neoplasm.