In the mean while, to further explore the possible mechanism of anti-AEG-1 ScFv on tumor growth inhibition in vivo, we also detected intracellular expression changes of AEG-1, Akt1,c-Myc, NF-ƘB and cleaved Caspase 3 as well as tumor cell apoptosis in Ad-sta-SFv treated tumor tissues. Here, CASP3 is linked to neoplasm.