GNAI1 and infection: In addition, these values were also very similar to those in experiment 2 (168 ± 17, Fig. 2B) where saline was not injected before the tongue protrusion test, indicating that AAV infection of the NAc and the resulting expression of Gi/Gq or intraperitoneal injection of saline 60 min prior to the test did not affect the number of basal tongue protrusions in response to honey.