We were also able to identify the heterozygous point mutation that is well known on 4EBP1 in HCT116 (a colorectal cancer cell line) [64] and quantitated levels of total and specific phosphorylation site of 4EBP1 (pS101) [61] using the same antibody designed to recognize unmodified region of the C-terminus. The gene discussed is EIF4EBP1; the disease is colorectal cancer.