In three unrelated studies, a total of 804 sera from myositis patients and 786 control sera were investigated with a line blot containing Mi-2α, Mi-2β, TIF1γ, MDA5, NXP2, SAE1, Ku, PM-Scl100, PM-Scl75, Jo-1, SRP, PL-7, PL-12, EJ, OJ and Ro-52 on one test strip [EUROLINE Autoimmune Inflammatory Myopathies 16 Ag, EUROIMMUN, Lübeck, Germany].22,25,31 The specificities for the individual antigens were between 97–100%. The gene discussed is SAE1; the disease is myositis disease.