To determine the differences between expression level of HCSC markers in HCC and normal tissues, the mRNA levels of CD90, EpCAM, CD133, CD24, CD13, CD34, SOX9, ABCG2, CD44, ALDH1A1, ALDH3A1, CK19, SOX12, and CD47 in HCC and normal tissues were analyzed based on Oncomine, GEPIA and HCCDB database, respectively. This evidence concerns the gene ALDH1A1 and hepatocellular carcinoma.