To determine the differences between expression level of HCSC markers in HCC and normal tissues, the mRNA levels of CD90, EpCAM, CD133, CD24, CD13, CD34, SOX9, ABCG2, CD44, ALDH1A1, ALDH3A1, CK19, SOX12, and CD47 in HCC and normal tissues were analyzed based on Oncomine, GEPIA and HCCDB database, respectively. The gene discussed is PROM1; the disease is hepatocellular carcinoma.