NFKB1 and depressive symptom measurement: In the current study, we examined nuclear and cytoplasmic levels of NF-κB in the hippocampus of an LPS-induced depression mouse model and determined the expression levels of NF-κB and p-NF-κB in LPS-induced primary microglia to assess the effects of rhFGF21 on the NF-κB signaling pathway.