CaM levels in the membranes of HD T cells were 51% higher than in the cytoplasm (with a membrane: cytoplasm CaM fluorescence ratio of 1.5); in HNSCC T cells they were 10% lower (membrane: cytoplasm CaM fluorescence ratio of 0.9) (Figure 2D). Here, CALM3 is linked to head and neck squamous cell carcinoma.