CASP1 and colitis: mRNA expression of pro-inflammatory cytokines in colon tissues as assessed by RT-qPCR was increased in Slco2a1−/− compared to WT mice on days 3 and 7 of DSS treatment (Fig. 3a)., Although Casp1 mRNA expression was decreased (Fig. 3b), protein levels of pro- and cleaved IL-1β, cleaved caspase-1 (CASP1), and NLRP3 were also significantly elevated in Slco2a1−/− compared to WT mice with colitis (Fig. 3c, Supplementary Fig. S3).