Cells with a high PPAT/GLS1 ratio such as BT-549 breast cancer cells (Fig. 5b), TGW neuroblastoma cells (Fig. 5c), and U-251 MG glioblastoma cells (Fig. 5d) were sensitive to PPAT depletion or GLS1 overexpression (Supplementary Fig. 9) with regard to their anchorage-independent growth. The gene discussed is GLS; the disease is glioblastoma.