As a culture media, we tested the standard culture media supplemented with EGF, R-spondin-1, Noggin, Jagged-1, and Rho-associated, coiled-coil containing protein kinase (ROCK) inhibitor Y27632, which we have confirmed robustly applicable to organoid culture of murine primary cells from various organs and human gynecological neoplasms [20,22,23,30,32], to facilitate future cross-referencing of organoids from various organs. This evidence concerns the gene EGF and female reproductive system neoplasm.