Finally, by comparing CCA to the group containing patients with other diseases (PSC and UC) and healthy individuals, the mRNA metallothionein 1F (MT1F), glutathione peroxidase 3 (GPX3), and lactate dehydrogenase 4 (LDH4) stood out as the ones with the best AUC values (0.915, 0.897, and 0.894, respectively), along with the ncRNAs U11 small nuclear (RNU11), LOC257358, and vault RNA 1-1 (VTRNA1-1) (0.830, 0.812, and 0.777, respectively) (Figure 7C), therefore constituting promising urine EV biomarkers for the accurate diagnosis of CCA. This evidence concerns the gene VTRNA1-1 and cholangiocarcinoma.