LGR5 and malignant colon neoplasm: First, de Sousa e Melo et al. used mouse-derived colon cancer organoid cultures (Apcmin; KrasG12V; p53CRIPSR; SMAD4CRIPSR) that expressed EGFP (enhanced green fluorescent protein) and the diphtheria-toxin (DT) receptor (DTR) under the control of the leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) promoter to initiate tumors in recipient mice [17].