SAMHD1 and infection: We also induced MDDCs maturation through productive infection of iMDDCs with vesicular stomatitis virus-pseudotyped HIV-1 expressing GFP (VSV-G–HIV-1–GFP), delivered together with virus-like particle carrying the HIV-2 protein Vpx, which allows SAMHD1 degradation thereby facilitating HIV RNA reverse transcription, innate sensing by cGAS, type I interferon production and subsequent MDDC maturation (Manel et al., 2010; Figure 3B).