A clinical factors which were linked to depression in diabetic patients were presence of complication [12], co-morbidities, duration of treatment and treatment with insulin [10], duration of illness more than 10 years [15], limb problems [16], presence of ≥ 3 diabetic complications, and negative life events and poor social support [17], total number of life events were statistically significant predictors of depression in diabetes patient [18]. This evidence concerns the gene INS and depressive symptom measurement.