In conclusion, this study aimed to identify hub genes involved in HCC by WGCNA of data from the TCGA database and concluded that 13 functional genes (GTSE1, PLK1, NCAPH, SKA3, LMNB2, SPC25, HJURP, DEPDC1B, CDCA4, UBE2C, LMNB1, PRR11, and SNRPD2) are highly differentially expressed in tumor and non-tumor tissues. This evidence concerns the gene SPC25 and hepatocellular carcinoma.