In the present study, C. butyricum significantly decreased the expression level of the pro-inflammatory cytokine (IL-1β and IL-8) production in intestines and the expression level of the pro-inflammatory cytokine (IFN-γ, IL-1β, IL-8, and TNF-α) in intestinal epithelial cells of chickens after Salmonella infection. The gene discussed is IFNG; the disease is Salmonella Infections.