Leveraging this technique, we demonstrated in our prior study that EVs exhibiting distinctive tetraspanin profiles are secreted by different glioma and other cancer cell lines, and that CD63+ EVs as well as double positive CD63+/CD81+ EVs and CD9+/CD63+ EVs are enriched in glioblastoma patient plasma samples [10]. This evidence concerns the gene CD9 and glioblastoma.