Importantly, however, the total count of double positive FASN+/CD63+ EVs (mean 4.1 × 105/mL GBM vs 5.8 × 104/mL HD, P = 0.001) as well as the total count of FASN+/CD81+ EVs (mean 8.0 × 105/mL GBM vs 2.3 × 105/mL HD, P = 0.007) was significantly increased in the plasma of glioblastoma patients (Figure 4C). This evidence concerns the gene CD81 and glioblastoma.