Here, we investigated genome-wide methylation patterns and genomic CNVs in freshly sorted and long-term cultured and sorted integrin alpha-6 (ITGA6) positive human testicular cells (n = 4 cultures) and compared these methylation patterns to primary seminoma tumors (n = 3), focusing on genome-scale DNA patterning as well as known oncogenic regions. The gene discussed is ITGA6; the disease is seminoma.