In vitro, ectopic overexpression of SPRED1 suppressed ERK signaling and the proliferation of AML cells, which was accompanied the accumulation of cells at S phase and induction of cell apoptosis; in contrast, silencing of SPRED1 activated ERK signaling, enhanced the proliferation and reduced the apoptosis of AML cells. Here, SPRED1 is linked to acute myeloid leukemia.