To verify the regulation of to autophagy by AMPK in bladder cancer cells, ULK1 phosphorylation and the levels of the autophagic markers (LC3 and p62) were determined in 5637 cells with AMPKα knockdown (sh-AMPKα) or overexpression (AMPKα-OE). The gene discussed is ULK1; the disease is urinary bladder cancer.