To this end, either 300 PD-1+, PD-1+ Tim3– Slamf6+ CD39–, or PD-1+ Tim3– Slamf6+ CD39+ OT1 CD8 TILs cells were isolated from B16.OVA tumors 21 days post-tumor engraftment following the gating strategy of Supplementary Figure 3A and transferred to a secondary host that was infected on the same day with OVA-expressing Listeria monocytogenes (Lm-OVA). Here, PDCD1 is linked to neoplasm.