PDCD1 and melanoma: To identify surface markers of tumor-specific Tcf1+ PD-1+ Tpe that would allow their selection for ACT, we performed a differential gene expression analysis of Tcf7+ Pdcd1+ (Tpe) vs. Tcf7- Pdcd1+ (Tex) from scRNAseq data of CD8 TILs from murine B16 (18) and human primary melanoma tumors [publicly available data from Sade-Feldman et al. (16)] (Supplementary Tables 1, 2).