After recognizing bacterial infection, TLRs activated MST1/2, which phosphorylated PKC-α and activated PKC-α-LyGDI (Rho-specific guanine nucleotide dissociation inhibitor)-Rac axis, promoting assembly of the TRAF6-ECSIT complex and ROS production (61) (Figure 5). This evidence concerns the gene ECSIT and bacterial infectious disease.