Using this method, we identified that the differentially expressed proteins between sham and ICH were associated with 13 prominent signaling pathways, which were blood coagulation, plasminogen activating cascade, p53 pathway, EGF receptor signaling pathway, FGF signaling pathway, cytoskeletal regulation by Rho GTPase, angiogenesis, integrin signaling pathway, Parkinson’s disease, Huntington disease, dopamine receptor mediated signaling pathway, T cell activation, and Wnt signaling. The gene discussed is TP53; the disease is juvenile Huntington disease.