To dissect a potential contribution of presenilin FAD mutations on AD pathology in our 3D AD cellular models, we also generated human neural stem cells that overexpress presenilin 1 with ΔE9 familial mutation alone (ReN-m-PS1ΔE9), and compared the impact of PS1ΔE9 on tau pathology in our 3D cellular model. Here, MAPT is linked to Alzheimer disease.