CD274 and neoplasm: Figure 2a shows that the secreted iPDL1 (PD-1-IgG Fc) bound to PD-L1 on the virus-infected (RFP positive) tumor cells, as well as uninfected (RFP negative) tumor cells in autocrine and paracrine manners by flow cytometry staining with an anti-IgG Fc to detect the binding of secreted iPDL1 to PD-L1 on tumor cells.