The percentage of PD-L1+ VV-iPDL1/GM-infected (RFP+) or uninfected (RFP−) tumor cells was significantly lower than that of VV-RFP-infected (RFP+) or uninfected (RFP−) tumor cells (Fig. 2b), suggesting that the binding of the iPDL1 secreted from VV-iPDL1/GM-infected cells to PD-L1 on tumor cells partially blocked PD-L1 staining with an PD-L1 antibody. The gene discussed is CD274; the disease is neoplasm.