Analysis of DT-treated CRC Lgr5eGFP organoids, in which Lgr5+ CSCs are selectively depleted, and post-sort confocal microscopy on untreated CRC Lgr5eGFP organoids confirmed that our gating strategy allowed to reliably isolate Lgr5+ and Lgr5− cancer cells (Figures S1N and S1O). The gene discussed is LGR5; the disease is colorectal carcinoma.