To compare the efficiency of Lgr5− cancer cells and Lgr5+ CSCs to initiate metastases, we injected equal amounts of sorted Lgr5+ CSCs and Lgr5− cancer cells into the mesenteric vein, which are transported through the portal vein to the liver. Four weeks later we found that, in addition to Lgr5+ CSCs, Lgr5− cancer cells were able to form metastases that displayed similar morphology, though with slightly lower efficiency (Figures 3K–3O; Figures S3J and S3K; n = 4 mice). The gene discussed is LGR5; the disease is cancer.