HBEGF and cancer: Just before the humane endpoint, we isolated the tumorigenic colons of these mice and established primary CRC organoids that consist of cancer cells expressing RFP-Confetti and of CSCs that additionally express Lgr5-driven eGFP and diphtheria toxin receptor (DTR) (referred as CRC Lgr5eGFP organoids; Figures S1A and S1B).