PHB1 and ovarian cancer: To examine whether c-Kit may phosphorylate PHB at Y259 to recruit Notch3 in the membrane raft to facilitate Notch3-mediated signaling in ovarian cancer, we performed a coimmunoprecipitation assay using anti-HA antibodies and found that not only c-Kit but also Notch3 were coimmunoprecipitated with raft-PHB (Fig. 5f).