This study resulted in several important findings, namely: (1) probiotic strains used in this study differentially induced macrophage pro-inflammatory cytokines in a subset- and strain-specific manner, (2) phagocytosis of probiotic was both macrophage subset- and bacterial strain-specific, (3) probiotic mix abrogates DMH-induced shortening of the colon and exhibits a beneficial effect on leukocyte count and colon tumour development, and (4) probiotic mix induces secretion of pro-inflammatory IL-18 by tumour tissue. This evidence concerns the gene IL18 and colonic neoplasm.