This study detected the expression levels of circ‐PGAM1, miR‐542‐3p, CDC5L, and PEAK1 in ovarian cancer tissues, validated the interaction between circ‐PGAM1 and miR‐542‐3p, between miR‐542‐3p and CDC5L, and between CDC5L and PEAK1, investigated their regulatory functions in the malignant biological behaviors of ovarian cancer, and elucidated the underlying mechanisms to uncover novel targets for ovarian cancer treatment. This evidence concerns the gene PGAM1 and ovarian cancer.