To further study whether miR‐542‐3p inhibits CDC5L expression and plays a tumor‐suppressing role through targeted binding to the 3′‐UTR of CDC5L, an miR‐542‐3p‐overexpressing plasmid and CDC5L‐overexpressing plasmid or CDC5L 3′UTR‐deficient plasmid were co‐transfected into ovarian cancer cells to investigate their effects on malignant behaviors of ovarian cancer cells. The gene discussed is CDC5L; the disease is ovarian carcinoma.