In the current study, we confirmed the high specificity of BCL11B and GLG1 for EwS in a far larger and independent cohort of 133 EwS and 320 non-EwS from 11 differential diagnoses, including 118 samples from close morphological mimics such as DSRCT, neuroblastoma, and rhabdomyosarcoma. This evidence concerns the gene BCL11B and rhabdomyosarcoma.