Moreover, CD19+IL-10+Bregs can act to resolve pathogenic inflammation [48] including that associated with perturbation of the gut microbiota [49] and obesity [50] and these were found to be significantly increased in the MLN of male, but not female, d340 mice exposed to ES-62, a cohort that exhibited protection against HCD-induced gut pathology (Fig 7E and 7F). This evidence concerns the gene IL10 and obesity due to melanocortin 4 receptor deficiency.