BRAF mutation was more related with lymph node metastasis (p = 0.030), bowel wall invasion, mucin production (p = 0.010), tumor thrombus (p = 0.002), perineural invasion (p = 0.018), poor differentiation (p = 0.001) and MSI status including MSS/L and MSI-H. The gene discussed is BRAF; the disease is metastatic malignant neoplasm in the lymph nodes.