To determine whether ASBT inhibition can prevent development of NASH and fibrosis in mice, male C57Bl/6J mice were fed a choline-deficient L-amino-defined diet (CDAA) or a choline-sufficient control diet (CSAA) with or without an ASBTi for 22 weeks (Figure 1A). This evidence concerns the gene SLC10A2 and metabolic dysfunction-associated steatohepatitis.