However, in HO-1 siRNA-knockdown KCs, the protective effects of MF on LPS/D-GalN-induced FHF were reduced compared with those in HO-1 positive KCs, as indicated by the increased death rate, elevated ALT/AST activities, and enhanced TNF-α production (Figures 6A–D, p < 0.01). This evidence concerns the gene TNF and TNF receptor 1-associated periodic fever syndrome.