Altogether these data support the contention that ACBP/DBI neutralization results in lipo-catabolic reactions, commensurate with the observation that ACBP/DBI neutralization resulted in a reduction of fat mass in multiple different models, including age-associated weight gain of mice kept on a normal diet, high-fat diet-induced obesity, as well as genetically determined obesity of leptin-deficient Ob/Ob mice [8]. This evidence concerns the gene LEP and obesity due to melanocortin 4 receptor deficiency.