Real‐time PCR was firstly performed to confirm miRNA correlation with atherosclerosis, and computational analysis and luciferase assay were performed to explore the target of miR‐34c, Western blot, and real‐time PCR were also utilized to reveal the effect of whether high glucose (HG) and miR‐34c affect miR‐34c, HMGB1 levels, NF‐κB p65 and TNF‐α levels, and the role of miR‐34c on vascular smooth muscle cells (VSMCs) viability induced by HG. This evidence concerns the gene TNF and atherosclerosis.