The top 20 mutant genes with high mutation frequency found in 360 HCC patients from TCGA database included TP53, TTN, CTNNB1, MUC16, propagated, PCLO, APOB, RYR2, ND5, CSMD3, OBSCN, ABCA13, ARID1A, CACNA1E, LRP1B, XIRP2, ALMS1, SPTA1, RYR1 and HMCN1. The gene discussed is CACNA1E; the disease is hepatocellular carcinoma.