In the present study, 10 cases of HCC patients in Nanjing Drum Tower Hospital were sequenced by whole-exome sequencing, and highly frequent mutant genes, such as MUC4, HYDIN, CDC27, TTN26, COL6A6, SYNE1, NEB, OBSCN, NEB, HSPG2, FLG, DNAH17, ASPM, AHNAK2 and XIRP2, were also detected in the TCGA database. The gene discussed is FLG; the disease is hepatocellular carcinoma.