In the present study, 10 cases of HCC patients in Nanjing Drum Tower Hospital were sequenced by whole-exome sequencing, and highly frequent mutant genes, such as MUC4, HYDIN, CDC27, TTN26, COL6A6, SYNE1, NEB, OBSCN, NEB, HSPG2, FLG, DNAH17, ASPM, AHNAK2 and XIRP2, were also detected in the TCGA database. This evidence concerns the gene HSPG2 and hepatocellular carcinoma.