Then we assessed the expression of NID1 upon JQ1 treatment in AGS and HGC27 cells using WB and quantitative reverse-transcription real-time PCR (qRT-PCR), and confirmed that JQ1 significantly decreased both the mRNA and protein expression of NID1 in a seemingly dose-dependent manner in GC cells, consistent with the findings from RNA-seq data (Fig. 4g). This evidence concerns the gene NID1 and gastric cancer.