One non-canonical peptide, KYKDRTNILF, derived from the downstream ORF (dORF) of the melanoma stem-cell marker ABCB5 gene in 0D5P, was also found to be immunogenic in both autologous CD8+ TILs and CD8+ T cells from peripheral blood lymphocytes (PBLs) (Fig. 9a–c). The gene discussed is CD8A; the disease is melanoma.