Thirdly, siRNA knockdown of CCR1 and CCR2 in E0771 breast cancer cells had no significant effect on metabolism under normal culture conditions, but significantly inhibited glycolytic capacity and glycolytic reserve when malignant cells were exposed to FAK-depleted-CAF CM, but not WT-CAF CM (Supplementary Fig. 5h, Fig. 6d). This evidence concerns the gene CCR2 and breast cancer.