To validate the localization of MYR1 at the cyst wall of in vitro tissue cysts, the endogenous locus of MYR1 was epitope tagged at the C terminus with three copies of the HA tag (3×HA) in the PruΔku80strain, using CRISPR/Cas9 (Fig. 1A). The gene discussed is MYO1B; the disease is cyst.