Our data suggest a key role of proteins regulating ACTH signalling (MRAP), cholesterol levels (HSL) and intra-mitochondria availability (StAR and TSPO), as well as cholesterol enzymatic processing to corticosterone (CYP11a1, CYP21 and CYP11b1) in the disruption of adrenal responsiveness observed in conditions of MPRED-induced adrenal insufficiency. This evidence concerns the gene CYP21A2 and Adrenal insufficiency.