To study the regulation of hyperglycemia on placental amino acid transporter and mTORC1 activity in vitro, human trophoblast cell line JEG-3 was cultured with different concentrations of glucose, and expression of LAT1, LAT2, p-4EBP1(Thr-37/46), and p-S6k1(Thr-389) was examined by Western blot. This evidence concerns the gene RPS6KB1 and Hyperglycemia.