The effect of NZ was pleiotropic: particularly in association with doxorubicin, it increased the amount of intratumor necrotic areas, as observed in hematoxylin-eosin staining, reduced the tumor cell proliferation, indicated by lower positivity for Ki67, and increased the cleaved caspase 3, i.e., the caspase-activated during apoptosis (Figure 5C,D). The gene discussed is CASP3; the disease is neoplasm.