HMGB1 and ischemia: Among the subcellular organelles, there was no merged image of HMGB1 on Rab5, Rab7, LAMP1, LC3A/B, and KDEL indicating that HMGB1 was not imported into the endosomes, phagosomes, lysosome, or endoplasmic reticulum in neurons at 6, 12, and 24 h after ischemia (Figure 2).