Moreover, in in vitro assays, several of the tested AMTs increased apoptosis of HT29 colon cancer cells, caused cell cycle arrest at G2/M phase, and/or suppressed migration and invasion, which were associated with downregulation of the ERK (extracellular signal regulated kinase), JNK (c JUN N terminal kinase) and AKT (protein kinase B (PKB) signaling pathways. The gene discussed is MAPK8; the disease is colonic neoplasm.