ACTA2 and Hepatic fibrosis: qPCR was used to verify RNAseq results of genes associated with hepatic fibrosis (Acta2; Col1a1), inflammation (Cd68) and steroid metabolism associated with cortisol (Cyp17a1), insulin signaling and glucose metabolism (Ppp1r3b; G6pc), and cell proliferation (aldo-keto reductase 1b8, Akr1b8, ortholog of AKR1B10) in female (Fig 9A) and male (Fig 9B) mice.